The Gambin group are interested in how certain proteins clump together, leading to cell death and Parkinson’s disease. 

They are using are a novel approach, which is much faster than traditional methods. They are using this new technique to solve different problems caused by proteins misbehaving in diseases affecting the brain, heart, muscle and other areas of the body. 

Research

The group’s research is focused on four areas:

Protein aggregation – studying how proteins cluster together to signal more efficiently and how that is link to pathologies and protein polymerization.

Aggregation analysis by single molecule techniques – studying protein aggregation has evolved based on new developments in single molecule spectroscopy. The group have developed a hybrid method that allows co-aggregation events. 

Aggregation in neurodegenerative diseases – one of the hallmarks of many neurodegenerative diseases are misfolding and pathological aggregation of proteins.  The formation of protein aggregates and plaques in Parkinson’s and Alzheimer’s disease is known to create havoc in cells. 

Prion-like behaviour in innate immunity – It has been recently discovered that two protein adaptors, known as ASC and MAVS, can create protein aggregates in immune cells with prion-like behaviours. Rather than be undesirable, this process can help amplify the immune response. 

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  • Prion-like behaviour in innate immunity
  • Aggregation analysis by single molecule techniques
  • Aggregation in neurodegenerative diseases
  • Protein aggregation and prion-like behaviour
Authors
Title
Published In
Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y, Alexandrov K.
Performance benchmarking of four cell-free protein expression systems.
Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814. [Epub ahead of print] PMID: 26301602
Polinkovsky ME, Gambin Y, Banerjee PR, Erickstad MJ, Groisman A, Deniz AA.
Ultrafast cooling reveals microsecond-scale biomolecular dynamics.
Nat Commun. 2014 Dec 17;5:5737. doi: 10.1038/ncomms6737. PMID: 25517430
Martin S, Papadopulos A, Tomatis VM, Sierecki E, Malintan NT, Gormal RS, Giles N, Johnston WA, Alexandrov K, Gambin Y, Collins BM, Meunier FA.
Increased polyubiquitination and proteasomal degradation of a Munc18-1 disease-linked mutant causes temperature-sensitive defect in exocytosis.
Cell Rep. 2014 Oct 9;9(1):206-18. doi: 10.1016/j.celrep.2014.08.059. Epub 2014 Oct 2. PMID: 25284778
Sierecki E, Stevers LM, Giles N, Polinkovsky ME, Moustaqil M, Mureev S, Johnston WA, Dahmer-Heath M, Skalamera D, Gonda TJ, Gabrielli B, Collins BM, Alexandrov K, Gambin Y.
Rapid mapping of interactions between Human SNX-BAR proteins measured in vitro by AlphaScreen and single-molecule spectroscopy.
Mol Cell Proteomics. 2014 Sep;13(9):2233-45. doi: 10.1074/mcp.M113.037275. Epub 2014 May 27. PMID: 24866125
Brooks AJ, Dai W, O'Mara ML, Abankwa D, Chhabra Y, Pelekanos RA, Gardon O, Tunny KA, Blucher KM, Morton CJ, Parker MW, Sierecki E, Gambin Y, Gomez GA, Alexandrov K, Wilson IA, Doxastakis M, Mark AE, Waters MJ.
Mechanism of activation of protein kinase JAK2 by the growth hormone receptor.
Science. 2014 May 16;344(6185):1249783. doi: 10.1126/science.1249783. PMID: 24833397
Han SP, Gambin Y, Gomez GA, Verma S, Giles N, Michael M, Wu SK, Guo Z, Johnston W, Sierecki E, Parton RG, Alexandrov K, Yap AS.
Cortactin scaffolds Arp2/3 and WAVE2 at the epithelial zonula adherens.
J Biol Chem. 2014 Mar 14;289(11):7764-75. doi: 10.1074/jbc.M113.544478. Epub 2014 Jan 27. PMID: 24469447
Gambin Y, VanDelinder V, Ferreon AC, Lemke EA, Groisman A, Deniz AA.
Visualizing a one-way protein encounter complex by ultrafast single-molecule mixing.
Nat Methods. 2011 Mar;8(3):239-41. doi: 10.1038/nmeth.1568. Epub 2011 Feb 6. PMID: 21297620